菝葜

更新时间: 2024-09-26 20:52:30 责编：网友投稿浏览

菝葜用途与合成方法

生物活性

Smilagenin (SMI) 是一种脂溶性小分子甾体皂苷元，来自知母 Rhizoma anemarrhenae 和天竺葵 Radix asparagi，广泛用于慢性神经退行性疾病的研究。Smilagenin (SMI) 通过增加毒蕈碱受体亚型 1 (M1)-受体的的密度来改善老年大鼠的记忆。Smilagenin (SMI) 通过刺激脑源性神经营养因子的基因表达减弱 Aβ (25-35) 诱导的神经变性，可用于 AD 的研究。

靶点

Human Endogenous Metabolite

体外研究

Smilagenin (10 μM; 24 hours) increases SH-SY5Y cell survival compared with Aβ(25-35) intoxicated cells. Smilagenin (10 μM; 24 hours) increases neurotrophic factor (GDNF) and neurotrophic factor (BDNF) mRNA level by promoting CREB phosphorylation in 1-methyl-4-phenylpyridimium (MPP + ) treated SH-SY5Y cells.

Cell Viability Assay

Cell Line:	SH-SY5Y cells
Concentration:	10 μM
Incubation Time:	24 hours
Result:	Elevated the SH-SY5Y cell viability.

RT-PCR

Cell Line:	SH-SY5Y cells
Concentration:	10 μM
Incubation Time:	24 hours
Result:	Increased GDNF and BDNF transcription.

体内研究

Smilagenin (intragastric administration; 10 or 26 mg/kg, once daily; 60 days) prevents the impairment of dopaminergic neurons in chronic MPTP/probenecid-induced mouse model.

Animal Model:	MPTP/probenecid-induced mouse model
Dosage:	10 or 26 mg/kg
Administration:	Intragastric administration; 10 or 26 mg/kg; once daily; 60 days
Result:	Ameliorated locomotor ability of MPTP/probenecid-lesioned mice.